Proteomics

Proteins catalyze and regulate biological processes making them attractive drug targets and diagnostic markers (Biomarkers). Proteomics is a very powerful and promising discipline studying the structure and function of proteins and their interactions with each other in biological processes.
Sandor Proteomics provides a comprehensive range of unbiased approaches to comparative proteomics supporting protein identification, structural analysis, quantitative studies and interactions of proteins. We assist characterization of both targeted protein complexes and trace components in lysates and biological fluids.

2D ELECTROPHORESIS
Protein separation and profiling

HIGH PRESSURE LIQUID CHROMATOGRAPHY
Amino acid analysis
Size exclusion
Reverse phase separation and purification

GAS CHROMATOGRAPHY- MASS SPECTROSCOPY

LC-MALDI TOF/TOF
Intact Protein Analysis
Peptide Mass Fingerprinting
Peptide Sequencing and PTM Analysis
Components identification in partially pure sample by LC-MALDI

X-RAY DIFFRACTION FACILITY
X-RAY Diffraction for Protein Crystals

CIRCULAR DICHROISM (CD) SPECTROPOLARIMETER
Analysis of secondary and tertiary structure of proteins & conformational changes
Optical purity determination
Nucleic acid structure determination and changes upon binding or melting
Thermodynamic parameters from temperature dependencies of CD signals

BIACORE
SPR/MS
Kinetics of binding
Solution affinity
Steady state affinity
Yes/no binding
Ligand fishing
Affinity ranking
Activity assays
Protein concentration
Thermo dynamics
Immunogenicity studies